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Microscopy|5 Article(s)
Sidelobe suppression in light-sheet fluorescence microscopy with Bessel beam plane illumination using subtractive imaging
Suhui Deng, Yiping Xiao, Jie Hu, Jianfang Chen, Yuhao Wang, and Mingping Liu
The fluorescence from the out-of-focus region excited by the sidelobes of a Bessel beam is the major concern for light-sheet fluorescence microscopy (LSFM) with Bessel beam plane illumination. Here, we propose a method of applying the subtractive imaging to overcome the limitation of the conventional LSFM with Bessel beam plane illumination. In the proposed method, the sample is imaged twice by line scanning using the extended solid Bessel beam and the ring-like Bessel beam. By subtracting between the two images with similar out-of-focus blur, the improved image quality with the suppression of the Bessel beam sidelobes and enhanced sectioning ability with improved contrast are demonstrated. The fluorescence from the out-of-focus region excited by the sidelobes of a Bessel beam is the major concern for light-sheet fluorescence microscopy (LSFM) with Bessel beam plane illumination. Here, we propose a method of applying the subtractive imaging to overcome the limitation of the conventional LSFM with Bessel beam plane illumination. In the proposed method, the sample is imaged twice by line scanning using the extended solid Bessel beam and the ring-like Bessel beam. By subtracting between the two images with similar out-of-focus blur, the improved image quality with the suppression of the Bessel beam sidelobes and enhanced sectioning ability with improved contrast are demonstrated.
Chinese Optics Letters
- Publication Date: Nov. 10, 2018
- Vol. 16, Issue 11, 111801 (2018)
Fast pixel shifting phase unwrapping algorithm in quantitative interferometric microscopy
Liang Xue, Shouyu Wang, Keding Yan, Nan Sun, Zhenhua Li, and Fei Liu
Quantitative interferometric microscopy is an important method for observing biological samples such as cells and tissues. As a key step in phase recovery, a fast phase unwrapping algorithm is proposed. By shifting mod 2\pi wrapped phase map for one pixel, then multiplying the original phase map and the shifted one, the phase discontinuities could be easily determined with high speed and efficiency. The method aims at enhancing phase retrieving efficiency without any background knowledge. We test our algorithm with both numerical simulation and experiments, by focusing our attentions on wrapped quantitative phase maps of cells. The results indicate that this algorithm features fast, precise and reliable. Quantitative interferometric microscopy is an important method for observing biological samples such as cells and tissues. As a key step in phase recovery, a fast phase unwrapping algorithm is proposed. By shifting mod 2\pi wrapped phase map for one pixel, then multiplying the original phase map and the shifted one, the phase discontinuities could be easily determined with high speed and efficiency. The method aims at enhancing phase retrieving efficiency without any background knowledge. We test our algorithm with both numerical simulation and experiments, by focusing our attentions on wrapped quantitative phase maps of cells. The results indicate that this algorithm features fast, precise and reliable.
Chinese Optics Letters
- Publication Date: Jun. 20, 2014
- Vol. 12, Issue 7, 071801 (2014)
Tuning the face orientation of ZnO nano/microcrystals by a wet chemical method
Aparna Thankappan, Sheenu Thomas, and V. P.
We successfully synthesize four kinds of ZnO nano/microcrystals including dumbbell microrods, nanoflakes, nanoplates, and microrods by a simple wet chemical method. Growth duration is found to play a crucial role in the morphologies of these ZnO nano/microcrystallites. In addition, growth conditions are system-atically studied as a function of precursor concentration and temperature. The structural and optical characteristics of the ZnO samples are further investigated by X-ray diffraction, scanning electron microscopy, and photoluminescence spectroscopy. We successfully synthesize four kinds of ZnO nano/microcrystals including dumbbell microrods, nanoflakes, nanoplates, and microrods by a simple wet chemical method. Growth duration is found to play a crucial role in the morphologies of these ZnO nano/microcrystallites. In addition, growth conditions are system-atically studied as a function of precursor concentration and temperature. The structural and optical characteristics of the ZnO samples are further investigated by X-ray diffraction, scanning electron microscopy, and photoluminescence spectroscopy.
Chinese Optics Letters
- Publication Date: Sep. 25, 2013
- Vol. 11, Issue 10, 101801 (2013)
Near-field lithography on the azobenzene polymer liquid crystal films
Douguo Zhang, Pei Wang, Yonghua Lu, Ming Bai, Jun Yang, Lin Tang, Jiangying Zhang, Hai Ming, Qijin Zhang, Jian Liu, Zebo Zhang, Li Cao, and Anlian Pan
In this article, we reported near-field research on azobenzene polymer liquid crystal films using scanning near-field optical microscopy (SNOM). Optical writing and subsequently topographic reading of the patterns with subwavelength resolution were carried out in our experiments. Nanometer scale dots and lines were successfully fabricated on the films and the smallest dot diameter is about 120 nm. The width of the line fabricated is about 250 nm. This method is also a choice for nanolithography. The mechanism of the surface deformation on the polymer films was briefly analyzed from the viewpoint of gradient force in the optical near field. The intensity distribution of the electric field near the tip aperture was numerically simulated using finite-difference time-domain (FDTD) method and the numerical simulation results were consistent with the experimental results. In this article, we reported near-field research on azobenzene polymer liquid crystal films using scanning near-field optical microscopy (SNOM). Optical writing and subsequently topographic reading of the patterns with subwavelength resolution were carried out in our experiments. Nanometer scale dots and lines were successfully fabricated on the films and the smallest dot diameter is about 120 nm. The width of the line fabricated is about 250 nm. This method is also a choice for nanolithography. The mechanism of the surface deformation on the polymer films was briefly analyzed from the viewpoint of gradient force in the optical near field. The intensity distribution of the electric field near the tip aperture was numerically simulated using finite-difference time-domain (FDTD) method and the numerical simulation results were consistent with the experimental results.
Chinese Optics Letters
- Publication Date: Jan. 01, 2005
- Vol. 3, Issue 2, 02107 (2005)
Cross-talk correction in dual-labeled fluorescent microarray scanning
Liqiang Wang, Zukang Lu, and Xuxiang Ni
Cross-talk phenomenon in dual-labeled fluorescent microarray scanning is analyzed from cross-excitation and cross-emission. It is turned out that the spectral overlap of the fluorophores is crucial for cross-talk error, and this error can be corrected by an image subtraction method. The experiment was successfully applied to separate the Cy3 channel and the Cy5 channel in microarray scanning. The cross-talk error was reduced from more than 1% to about 0.1%. Cross-talk phenomenon in dual-labeled fluorescent microarray scanning is analyzed from cross-excitation and cross-emission. It is turned out that the spectral overlap of the fluorophores is crucial for cross-talk error, and this error can be corrected by an image subtraction method. The experiment was successfully applied to separate the Cy3 channel and the Cy5 channel in microarray scanning. The cross-talk error was reduced from more than 1% to about 0.1%.
Chinese Optics Letters
- Publication Date: Jan. 01, 2004
- Vol. 2, Issue 3, 03162 (2004)
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