Wavefront aberration can well reflect the performances of optical system. The test result of wavefront is usually expressed in a linear combination of Zernike polynomials. The wavefront of the transmission optical system needs to be tested at specific wavelengths. From now on, only a few wavelength wavefronts can be accurately tested because of the limitation of detection instrument. A new idea is put forward in this paper, the wavefront can be tested at any wavelength by analyzing the functional relationship between transmitted wavefront Zernike coefficients and wavelength, which can indirectly reflect the variation of the wavefront with the wavelength. The optical system is modeled by Zemax. The wavefront Zernike coefficients of the optical system at different wavelengths are collected. Then the possible function form of the curve of the Zernike coefficients and the wavelength are fitted by Matlab curve fitting tool. Finally, the Conrady-Zernike formula is determined. The maximum error of the calculated Zernike coefficients is within 1%, which is calculated by the Conrady-Zernike formula in the simulated optical system. The results show that the wavefront Zernike coefficients and the wavelength of transmitted optical system are basically consistent with the Conrady-Zernike formula.