Lixin Liu, Meiling Zhang, Zhaoqing Wu, Qianqian Yang, Peng Gao, Ping Xue. Application of Adaptive Optics in Fluorescence Microscope[J]. Laser & Optoelectronics Progress, 2020, 57(12): 120001
- Laser & Optoelectronics Progress
- Vol. 57, Issue 12, 120001 (2020)
![Structure of typical adaptive optical system[19]](/richHtml/lop/2020/57/12/120001/img_1.jpg)
Fig. 1. Structure of typical adaptive optical system[19]
![AO wide-field microscope system and comparison of wide-field images before and after AO correction[23]. (a) Schematic of AO wide-field microscope system based on SHWS, fluorescent beads, and DM; (b) wide-field images of green fluorescent beads before and after AO correction](/richHtml/lop/2020/57/12/120001/img_2.jpg)
Fig. 2. AO wide-field microscope system and comparison of wide-field images before and after AO correction[23]. (a) Schematic of AO wide-field microscope system based on SHWS, fluorescent beads, and DM; (b) wide-field images of green fluorescent beads before and after AO correction
![Conjugate AO wide-field microscope system and comparison of images before and after AO correction. (a) Schematic of PAW-based conjugate AO wide-field microscope system[26]; (b) fluorescence images of aberrated fluorescently-labeled mouse kidney section without and with conjugate AO correction[27]](/Images/icon/loading.gif){kind=icon}
Fig. 3. Conjugate AO wide-field microscope system and comparison of images before and after AO correction. (a) Schematic of PAW-based conjugate AO wide-field microscope system[26]; (b) fluorescence images of aberrated fluorescently-labeled mouse kidney section without and with conjugate AO correction[27]
Fig. 4. AOSIM system and comparison of images before and after AO correction[33]. (a) Schematic of woofer-tweeter AOSIM system; (b) wide field and SIM images of GFP-labeled aCC/RP2 motoneurons of a Drosophila embryo before and after AO correction
Fig. 5. Schematics of two AO single molecule location microscope systems. (a) Schematic of AO-PALM system based on WFS and DM[35]; (b) schematic of wavefront sensorless feedback AO single molecule switch microscope based on DM and image sharpness measurement[37]
Fig. 6. Aberration correction of microtubules by AO-STORM system based on search algorithm. (a) Aberration correction using GA-based AO-STORM system on microtubules of hepG2 cell[39]; (b) aberration correction using PSO-based AO-STORM system on microtubules of Hela cell[40]
Fig. 7. AO confocal microscope system and comparison of images before and after AO correction[42]. (a) Schematic of AO confocal fluorescence microscope based on SHWS, fluorescent beads, and DM; (b) confocal fluorescence images through 100 μm thick mouse brain tissue before and after AO correction
Fig. 8. Schematic of wavefront sensorless AO confocal fluorescence microscope based on DM and SPGD algorithm[46]
Fig. 9. Schematic of DAO confocal microscope based on guide star hologram[52]
Fig. 10. Aberration measurement by confocal microscope[53]. (a) Pupil segmentation on SLM; (b) interference patterns generated by focusing light from two selected circular segments
Fig. 11. AO two-photon microscope system and comparison of images before and after AO correction[57]. (a) Schematic of AO two-photon microscope based on SHWS and open-loop control; (b) two-photon images of live Drosophila embryo at depth of 51 μm before and after AO correction
Fig. 12. Schematics of two AO-STED microscope systems. (a) Schematic of AO-STED system using two SLMs for aberration correction[62]; (b) schematic of AO-STED system using DM and SLM for aberration correction[63]
Fig. 13. Annular focal spots[66]. (a) Before aberration compensation; (b) after aberration compensation
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