The details of cross-sectional images based on Fourier domain optical coherence tomography play an important role that is limited to nonuniform sampling, spectral dispersion, inverse discrete Fourier transform (IDFT), and noise. In this section, we propose a method for emphasizing axial details to the greatest extent possible. After removing spectral dispersion, uniform discretization in the wavenumber domain is performed based on two interferograms via a specified offset in depth, with no spectrum calibration. The sampling number in IDFT is optimized to improve axial sensitivity up to 1.62 dB. The proposed process has the advantage of being based on numerical computation rather than hardware calibration, which benefits cost, accuracy, and efficiency.