500 μm field-of-view probe-based confocal microendoscope for large-area visualization in the gastrointestinal tract

Hua Li; Zhengyi Hao; Jiangfeng Huang; Tingting Lu; Qian Liu; Ling Fu

doi:10.1364/PRJ.431767

Journals >Photonics Research >Volume 9 >Issue 9 >Page 1829 > Article

Photonics Research
Vol. 9, Issue 9, 1829 (2021)

500 μm field-of-view probe-based confocal microendoscope for large-area visualization in the gastrointestinal tract

Hua Li^1、2, Zhengyi Hao^1、2, Jiangfeng Huang^1、2, Tingting Lu^1、2, Qian Liu^1、2、3, and Ling Fu^{1、2、3、*}

Author Affiliations

¹Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China

²MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan 430074, China

³School of Biomedical Engineering, Hainan University, Haikou 570228, China

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DOI: 10.1364/PRJ.431767 Cite this Article Set citation alerts

Hua Li, Zhengyi Hao, Jiangfeng Huang, Tingting Lu, Qian Liu, Ling Fu. 500 μm field-of-view probe-based confocal microendoscope for large-area visualization in the gastrointestinal tract[J]. Photonics Research, 2021, 9(9): 1829 Copy Citation Text

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Abstract

Rapid large-area tissue imaging at the cellular resolution is important for clinical diagnosis. We present a probe-based confocal microendoscope (pCM) with a field-of-view (FOV) diameter over 500 μm, lateral resolution of 1.95 μm, and outer diameter of 2.6 mm, compatible with the biopsy channel of conventional gastroscopes. Compared to a conventional pCM, our system’s FOV increased by a factor of 4 while maintaining the probe size and cellular resolution—comparable to the FOV of Zeiss Axio Observer’s

20 \times / 0.8

numerical aperture objective lens. Ex vivo imaging of wide areas in healthy and diseased rat gastrointestinal tract tissues demonstrates the system’s practicality.