• Laser & Optoelectronics Progress
  • Vol. 60, Issue 11, 1106012 (2023)
Aoni Wei1, Chengbing Qin1、2、*, Shuai Dong1, Xinqin Meng1, Yunrui Song1, Xiangdong Li1, Xilong Liang1、3, Guofeng Zhang1, Ruiyun Chen1, Jianyong Hu1, Zhichun Yang1, Jianzhong Huo3, Liantuan Xiao1、2、**, and Suotang Jia1
Author Affiliations
  • 1State Key Laboratory of Quantum Optics and Quantum Optics Devices, Institute of Laser Spectroscopy, Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan 030006, Shanxi, China
  • 2College of Physics, Taiyuan University of Technology, Taiyuan 030024, Shanxi, China
  • 3Taiyuan Central Hospital of Shanxi Medical University, Taiyuan 030009, Shanxi, China
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    DOI: 10.3788/LOP230749 Cite this Article Set citation alerts
    Aoni Wei, Chengbing Qin, Shuai Dong, Xinqin Meng, Yunrui Song, Xiangdong Li, Xilong Liang, Guofeng Zhang, Ruiyun Chen, Jianyong Hu, Zhichun Yang, Jianzhong Huo, Liantuan Xiao, Suotang Jia. Research Progress of Super-Resolution Fluorescence Microscopy[J]. Laser & Optoelectronics Progress, 2023, 60(11): 1106012 Copy Citation Text show less

    Abstract

    Super-resolution fluorescence microscopy (SRFM), which can bypass the optical diffraction limit, provide an extremely important research tool for investigations of the subcellular structures and the dynamic processes of biological macromolecules. Therefore, since its invention, SRFM has been paid attention to and applied by scientists in the field of biology and medicine. Benefiting from the rapid development of life science and biotechnology in recent years, SRFM has been developed unprecedentedly. In this paper, we will mainly focus on the research progresses of four new kinds of SRFM, including minimal photon fluxes (MINFLUX), super-resolution optical fluctuation imaging (SOFI), super-resolution image based on anti-bunching effect, and deep-learning based super-resolution microscopy, respectively. We will briefly describe the recent research progresses and applications of these SRFM techniques from the basic principles, experimental implementation methods and related requirements, imaging performance, and comparison with other technologies, as well as the combination with other super-resolution techniques, to provide some references for researchers in this field.
    σ˜CRBquadx=1NL41+xL/22
    Irk,t=k=1Nδr-rk·εk·skt
    Fr,t=k=1Nhr-rk·εk·skt
    δFr,t=Fr,t-Fr,tt=khr-rk·εk·δskt
    Gnr,τ1,,τn-1=δFr,tδFr,t+τ1δFr,t+τn-1t=khnr-rk·εkn·δsktskt+τ1skt+τn-1
    Cnr,τ1,,τn-1=khnr-rk·εkn·wkr,τ1,,τn-1
    gn=i=0n-1N^-iN^n
    Ax=g20-1Nx2=QxNx
    hAx=hx2
    hnx=hxn
    Aoni Wei, Chengbing Qin, Shuai Dong, Xinqin Meng, Yunrui Song, Xiangdong Li, Xilong Liang, Guofeng Zhang, Ruiyun Chen, Jianyong Hu, Zhichun Yang, Jianzhong Huo, Liantuan Xiao, Suotang Jia. Research Progress of Super-Resolution Fluorescence Microscopy[J]. Laser & Optoelectronics Progress, 2023, 60(11): 1106012
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