nm by an optical fiber detection system">
  • Journal of Innovative Optical Health Sciences
  • Vol. 15, Issue 6, 2240011 (2022)
Cheng Zhang1, Hongxin Lin1、*, Ying Hu1, Jian Sui2, Lisheng Lin1、**, and Buhong Li1
Author Affiliations
  • 1Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, Fujian 350007, P. R. China
  • 2Department of Gastrointestinal Surgery, Fujian Provincial Hospital, Fuzhou, Fujian 350013, P. R. China
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    DOI: 10.1142/S1793545822400119 Cite this Article
    Cheng Zhang, Hongxin Lin, Ying Hu, Jian Sui, Lisheng Lin, Buhong Li. Monitoring of time-resolved singlet oxygen luminescence at 1270nm by an optical fiber detection system[J]. Journal of Innovative Optical Health Sciences, 2022, 15(6): 2240011 Copy Citation Text show less

    Abstract

    Singlet oxygen (1O2) is the main cytotoxic substance in Type II photodynamic therapy (PDT). The luminescence of 1O2 at 1270nm is extremely weak with a low quantum yield, making the direct detection of 1O2 at 1270nm very challenging. In this study, a set of highly sensitive optical fiber detection system is built up to detect the luminescence of photosensitized 1O2. We use this system to test the luminescence characteristics of 1O2 in pig skin tissue ex vivo and mouse auricle skin in vivo. The experimental results show that the designed system can quantitatively detect photosensitized 1O2 luminescence. The 1O2 luminescence signal at 1270nm is successfully detected in pig skin ex vivo. Compared with RB in an aqueous solution, the lifetime of 1O2 increases to 17.4±1.2μs in pig skin tissue ex vivo. Experiments on living mice suggest that an enhancement of 1O2 intensity with the increase of the TMPyP concentration. When the dose is 25mg/kg, the vasoconstriction can reach more than 80%. The results of this study hold the potential application for clinical PDT dose monitoring using an optical fiber detection system.Singlet oxygen (1O2) is the main cytotoxic substance in Type II photodynamic therapy (PDT). The luminescence of 1O2 at 1270nm is extremely weak with a low quantum yield, making the direct detection of 1O2 at 1270nm very challenging. In this study, a set of highly sensitive optical fiber detection system is built up to detect the luminescence of photosensitized 1O2. We use this system to test the luminescence characteristics of 1O2 in pig skin tissue ex vivo and mouse auricle skin in vivo. The experimental results show that the designed system can quantitatively detect photosensitized 1O2 luminescence. The 1O2 luminescence signal at 1270nm is successfully detected in pig skin ex vivo. Compared with RB in an aqueous solution, the lifetime of 1O2 increases to 17.4±1.2μs in pig skin tissue ex vivo. Experiments on living mice suggest that an enhancement of 1O2 intensity with the increase of the TMPyP concentration. When the dose is 25mg/kg, the vasoconstriction can reach more than 80%. The results of this study hold the potential application for clinical PDT dose monitoring using an optical fiber detection system.