Fig. 1. Principle of non-invasive deep focusing in random scattering medium. (a) Schematic diagram of non-invasive deep focusing in random scattering medium; (b) target speckle; (c) target sub-speckles

Fig. 2. Results of wave-front shaping based on region speckle variance. (a) Excitation speckle on CAM2 of non-optimized; (b) excitation speckle on CAM2 of optimized; (c) fluorescent speckle on CAM1 of non-optimized; (d) fluorescent speckle on CAM1 of optimized; (e) variation of intensity of excitation speckle on targets; (f) variation of intensity of target speckle; (g) variation of region variance

Fig. 3. Selection focusing based on region speckle variance. (a) Target speckle; (b) excitation speckle after area variance optimization of target sub-speckle #1; (c) excitation speckle after area variance optimization of different sub-speckles

Fig. 4. Evolution of speckle contrast

Table 1. Primary simulation parameters