Detection and imaging technology in a scattering medium is widely used in the biomedical field. However, the current technology still has some problems, such as low efficiency of external excitation or uncertain focus position. Thus, this paper proposed region variance feedback using the wavefront shaping method to realize non-invasive focusing deep in a scattering medium. Here, the region variance of the target speckle was used as the feedback of the wavefront shaping, and the excitation speckle in the scattering medium was focused on a single target by phase modulating with a spatial light modulator. The results show that the region variance method using the wavefront shaping realizes deep focusing on a single target in the scattering medium and selectively focuses on the determined target position according to the region selection. This selective focusing technology provides a new way for multiple targets imaging in biological tissue.