This study experimentally investigates the maximum measurable particle size of interferometric particle imaging (IPI). The maximum measurable particle size for IPI was examined in relation to the effect of object distance variation brought on by several object planes in the same field of vision. The IPI experimental system irradiated by a single beam was built to measure the polystyrene mixed particle fields with particle diameter of 51 μm and 110 μm, and the maximum measurable particle size in different collection areas in the same field of view was analyzed. The outcomes of the experiment demonstrate that the spatial relationship between the objects in the experimental system has an impact on the maximum quantifiable particle size of IPI technology. The maximum quantifiable particle size of various acquisition areas within the same field of view varies for an experimental system with fixed parameters.