Fig. 1. (a) The ophthalmic photoacoustic microscopy developed in this study. DM, dichroic mirror; ConL, condenser lens; FC, fiber coupler; SMF, single-mode fiber; OL, objective lens; UST, ultrasonic transducer; SO, silicone oil; EA, electrical amplifier; DAQ, Adata acquisition; ZCB, Zolix control box; PICC, Physik Instrumente control card; PC, personal computer. (b) The physical map of a mouse during imaging; Scale bar=10 mm.

Fig. 1. Photoacoustic imaging results of iris in 30 mice. Scale bar=1 mm.

Fig. 2. (a) The flow chart of vascular morphologic information analysis. (b) The flow chart of vascular functional imaging; Scale bar=0.5 mm.

Fig. 3. (a−b) The optical microscopic images of a healthy eyeball before and after burn. (c) The HE staining result of the eyeball after photoacoustic imaging. Scale bar=0.2 mm.

Fig. 4. (a−b) The MAP images of a representative mouse scanned by conventional OR-PAM and the new method developed in this study. (c−d). The depth encoded images of (a−b), respectively. Scale bar=0.5 mm.

Fig. 5. (a−j) The MAP images of all mice before and after burn. (k−o) Quantitative analysis the vascular information of all mice, including vascular diameter (k), density (l) and tortuosity (m−o). Scale bar=0.5 mm.

Fig. 6. The sO₂ maps of all mice before and after burn. Scale bar=0.5mm.