The effect of different ultrasound intensities from 0 to 0.8 W/cm² on the permeability and light penetration depth of glycerol solutions in human skin in vivo were investigated. The experiment used optical coherence tomography (OCT) imaging technology to perform quantitative continuous measurements. The results show that ultrasound has the ability to accelerate the penetration of glycerol solution and enhance the penetration of light through the skin with the intensity of pulsed ultrasound ranging from 0 to 0.8 W/cm², and the optical clearing effect of the skin increases with an increase in ultrasound intensity. The sample that was only applied with glycerol solution was defined as the control group, and the sample with the ultrasound effect and glycerol solution applications was defined as the experimental group. When the results of experimental and control groups were compared, it was observed that the average permeability coefficient of 15% glycerol solution in human skin treated with 0.2, 0.4, 0.6, 0.8 W/cm² pulsed ultrasound was increased by 2.6%, 9.5%, 14.7%, and 19.8%, respectively, compared to the samples with only 15% glycerol solution application. The average permeability coefficient of 30% glycerol solution treated with pulsed ultrasound in human skin was increased by 3.7%, 12%, 16.7%, and 22.2% respectively, compared to the control group that only used 30% glycerol solution. The conclusions suggested that the average permeability coefficient of glycerol solution increased with increasing pulsed ultrasonic intensity in the range of 0.2‒0.8 W/cm².