Meng-jun ZHANG, Li-li LIU, Xie-li YANG, Jing-fang GUO, Hao-yang WANG. Multispectral Analysis of Interaction Between Catechins and Egg Yolk Immunoglobulin and the Change of Bacteriostasis[J]. Spectroscopy and Spectral Analysis, 2022, 42(7): 2297
- Spectroscopy and Spectral Analysis
- Vol. 42, Issue 7, 2297 (2022)
Fig. 1. Ultraviolet-visible spectrum of different concentrations of catechins binding to IgY
The mass concentration of IgY was 0.5 mg·mL-1; The mass concentrations of 1 to 5 catechins were 0, 6.25, 12.5, 18.75, 25 μg·mL-1, respectively, as shown inFigure 2
The mass concentration of IgY was 0.5 mg·mL-1; The mass concentrations of 1 to 5 catechins were 0, 6.25, 12.5, 18.75, 25 μg·mL-1, respectively, as shown in
Fig. 2. Fluorescence spectra of different concentrations of catechins and different temperature binding to IgY
(a): 18 ℃; (b): 25 ℃; (c): 37 ℃
(a): 18 ℃; (b): 25 ℃; (c): 37 ℃
Fig. 3. Stern-Volmer plots of IgY quenched by catechinat different temperatures
Fig. 4. Logarithmic diagram of IgY-catechin complexes at different temperatures
Fig. 5. Fourier transform infrared spectra of IgY and IgY-catechins complex
Fig. 6. Antibacterial effect of IgY, mixture and IgY-catechin complex
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Table 1. Quenching rate constants and correlation coefficients of catechin and IgY
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Table 2. Apparent binding constant, number of binding sites and linear correlation coefficient of catechin-IgY complex
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Table 3. Thermodynamic parameters of catechin-IgY complex
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Table 4. The relative content of secondary structure of protein in amide Ⅰ band of IgY and IgY-catechin complex
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Table 5. Bacteriostatic rate of IgY, mixture and IgY-catechin complex
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