We establish a 210 km optical frequency signal transfer test link along the telecom provincial backbone optical fiber network between Xi’an and Xian Yang, and the loss of the link is 0.23 dB/km. A fiber interferometer based laser with linewidth of 200 Hz is used as the light source, and the laser is conveyable. Two bilateral erbium-doped optical fiber amplifiers (EDFAs) with low noise are used to compensate the fiber link loss and increase the transmission distance of signal. The average gain of amplifier is controlled at about 15 dB to avoid the self-excited oscillation. The additional phase noise of the fiber link under different conditions is measured and analyzed, and the regular disturbance caused by the railway vibration is observed. The phase noise in the link is suppressed by 23 dB when we use the noise suppression system. When the noise data from the railway vibration is eliminated, the second level frequency stability of the 210 km urban communication link can achieve 1.51×10-14 and be better than 5×10-17 at 104 s. The remote transfer test for optical frequency signal based on 210 km communication link is completed, and the main influence factors limiting the frequency stability are analyzed. The supplementary requirements are put forward for the current fiber setting way. The study provides theoretical supplement for achieving high accuracy optical frequency signal transmission and comparison based on the communication link.