Fig. 1. CARS. (a) Principle of CARS
[10] (Reproduced under the terms of the CC-BY license, Copyright 2014, Potcoava M C et al.); (b) schematic of CARS microscopy
[11](Reproduced with permission, Copyright 2018, Society of Photo‑Optical Instrumentation Engineers)
Fig. 2. Principle of SRS. (a) Energy diagram of SRS; (b) input and output spectra of SRS; (c) detection scheme of SRL; (d) setup of SRS microscopy
[12] (Reproduced with permission, Copyright 2008, American Association for the Advancement of Science)
Fig. 3. CARS images of 3T3-L1 cell culture at different time after adding induction media
[15]. (a) 0 h; (b) 24 h; (c) 48 h; (d) 60 h; (e) 96 h; (f) 192 h (Reproduced under the terms of the CC-BY license, Copyright 2003, American Society for Biochemistry and Molecular Biology )
Fig. 4. Comparison between SRS images and HES images of pancreatic tissue
[28]. (a) SRS image of healthy pancreatic tissue; (b) HES image of healthy pancreatic tissue; (c) SRS image of cancerous pancreatic tissue; (d) HES image of cancerous pancreatic tissue; (e) SRS image of the tumor area of pancreatic tissue; (f) HES image of the tumor area of pancreatic tissue (Reproduced under the terms of the CC-BY license, Copyright 2019, Sarri B et al.)
Fig. 5. Schematic diagram of photothermal microscopy
[31](Reproduced with permission, Copyright 2004, American Physical Society)
Fig. 6. Photothermal microscopy images. (a) COS-7 cells before digitonin addition
[32]; (b)(c) COS-7 cells after digitonin addition
[32](Reproduced under the terms of OSA Open Access Publishing Agreement, Copyright 2007, Optical Society of America); (d) single mitochondria
[33] (Reproduced with permission, Copyright 2010, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim); (e) HeLa cells
[34] (Reproduced under the terms of OSA Open Access Publishing Agreement, Copyright 2019, Optical Society of America); (f) melanoma cells
[35] (Reproduced under the terms of OSA Open Access Publishing Agreement, Copyright 2015, Optical Society of America); (g) oligodendrocytes
[36] (Reproduced with permission, Copyright 2019, American Chemical Society); (h) vesicular stomatitis virus
[37]; (i) poxvirus
[37] (Reproduced with permission, Copyright 2021, American Chemical Society)
Fig. 7. 3D images of photothermal microscopy. (a) Zebrafish gill larvae blood vessels
[38] (Reproduced with permission, Copyright 2010,American Institute of Physics); (b) melanoma
[39] (Reproduced under the terms of the CC-BY license, Copyright 2016,He J P et al.); (c) lipid droplets stored in PC-3 prostate cancer cells
[40] (Reproduced under the terms of the CC-BY license, Copyright 2016,Zhang Delong et al.)
Fig. 8. Schematic diagrams of SPRM. (a) Schematic diagram of wide-field SPRM; (b) schematic diagram of scanning SPRM
[43](Reproduced under the terms of OSA Open Access Publishing Agreement, Copyright 2012, Optical Society of America)
Fig. 9. SPRM imaging. (a) Single H1N1 influenza virus
[46]; (b) single T4 bacteriophage
[47] (Reproduced with permission, Copyright 2017, Society of Photo‑Optical Instrumentation Engineers); (c) single DNA molecular chain
[48] (Reproduced with permission, Copyright 2014, American Chemical Society)
Fig. 10. Study on molecular dynamic using SPRM. (a) 3D structure of microtubule
[51] (Reproduced with permission, Copyright WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim); (b)
z-axis motility profile of single bacteria
[52] (Reproduced with permission, Copyright 2016, American Chemical Society); (c) 2D tracking of single exosome
[53] Fig. 11. Study on cell-substrate interaction using SPRM. (a) Distribution of adhesion intensity of cell-substrate
[55]; (b) distribution of cell-substrate distance
[56]; (c) 3D reconstruction process of cell-substrate
[57] (Reproduced with permission, Copyright 2012,2014,2018, American Chemical Society)
Fig. 12. Schematic diagram of iSCAT
[62]. (a) Wide-field iSCAT; (b) fast beam-scanning iSCAT (Reproduced under the terms of the CC-BY-NC-ND license, Copyright 2021, Priest L et al.)
Fig. 13. iSCAT imaging of single virus and biomolecule. (a) Single SV40
[63]; (b) single streptavidin
[66] (Reproduced with permission, Copyright 2007,2017, American Chemical Society)
Fig. 14. Study on biomolecular dynamics using iSCAT. (a) Vesicle changes
[67](Reproduced with permission, Copyright 2013, American Chemical Society); (b) nanodomain formation
[68]; (c) myosin filament dynamics
[70](Reproduced under the terms of the CC-BY license, Copyright 2020, Biophysical Society)
Fig. 15. Study on structure of living cells using iSCAT
[71]. (a) Cell focaladhesions and filopodium structure;(b) cell edge structure (Reproduced under the terms of the CC-BY-NC license, Copyright 2018, The Royal Society of Chemistry)
Technique | Lateral resolution | Axial resolution | Laser power | Sensitivity | Temporal resolution | Imaging time | Ref |
---|
CRS | CARS | 600 nm | 1.1 µm | 14 mW(pump) 7 mW(Stokes) | - | 10 μs | ~1.6 s | [20] | SRS | 400 nm | 2 µm | 60 mW(pump) 100 mW(Stokes) | - | 2 μs | ~1 s | [27] | PTM | 290 nm | 860 nm | 0.9 mW(pump) 7 mW(probe) | 5 nm | 5 μs | several seconds | [34] | SPRM | Wide-field | 260 nm | - | 5 mW | 15 nm | 2 ms | 2 ms | [49,51] | Scanning | 186 nm | 2.3 nm | - | - | 100 ms | 73 min | [57] | iSCAT | Wide-field | ~190 nm | - | 10 mW | 65 ku | ~0.33 ms | ~0.33 ms | [64] | Fast beam-scanning | 1 μm | - | 2.5 kW/cm2 | 60 ku | - | ~100 µs | [65] |
|
Table 1. Performance comparison of four label-free optical microscopic imaging techniques