Yu LIU, Zeng-wei LI, Zhi-peng DENG, Qing-xian ZHANG, Li-kou ZOU. Fast Detection of Foodborne Pathogenic Bacteria by Laser-Induced Fluorescence Spectrometry[J]. Spectroscopy and Spectral Analysis, 2021, 41(9): 2817
- Spectroscopy and Spectral Analysis
- Vol. 41, Issue 9, 2817 (2021)
Fig. 1. Structure of portable 405 nm laser-induced fluorescence detector
Fig. 2. The instrument and operation flow chart
Fig. 3. P =10~100 mW, c =1.5OD, the fluorescence spectra of E.faecalis (a); and Relationship between Intensity and Power (b)
Fig. 4. Fluorescence spectra of bacteria samples
Fig. 5. Division of characteristic areas
Fig. 6. The standard cluster point of training set (a), sample cluster points of test set (b)
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Table 1. Parameters of the laser
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Table 2. Parameters of the spectrometer
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Table 3. P =50 mW, λ =405 nm, peak wavelength and relative intensity of bacteria samples
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Table 4. List of intrinsic fluorophores with relatively high fluorescence quantum efficiency along with their absorption and emission maxima[12]
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Table 5. Characteristic areas and values
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Table 6. Correct recognition rate of E.faecalis and S. Typhimurium by DC
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