How to effectively treat periodontitis and achieve regeneration of damaged periodontal tissue has always been a challenging problem in the treatment of periodontal diseases, and mineralization is one of the key factors in the normal periodontal development and function. The aim of this study was to investigate the effects of Li₂Ca₂Si₂O₇ bioceramics on proliferation and mineralization of human periodontal ligament fibroblasts (HPLFs) and possibility of its application in periodontal tissue regeneration. Li₂Ca₂Si₂O₇ ceramic powder was synthesized by Sol-Gel method. It was found that Li₂Ca₂Si₂O₇ powder had good ability of hydroxyapatite mineralization by immersing in simulated body fluid. The in vitro biological results showed that Li₂Ca₂Si₂O₇ powder extract could significantly promote proliferation of HPLFs in the concentration range of 3.125~25 mg/mL, and at low concentration (6.25 mg/mL), could significantly induce mineralization of HPLFs cells (p<0.05). All these experimental results reveal that the new Li₂Ca₂Si₂O₇ powder can promote proliferation and mineralization of HPLFs, and it may be a promising bioactive material for periodontal tissue regeneration.