To evaluate the jamming effect effectively, the research on the jamming influence of the laser high repetition which is made by interference factors of interfering frequency, wave door width, encoded mode and jamming moment was carried out. Firstly, three models of wave gate setting, coding identification and high repetition jamming were set up through deeply analyzing the anti-interference key technology and jamming mechanism. Secondly, a scheme was put forward, which was about judging the jamming signal in time gate and simulating the trajectory by the judge. Finally, based on the proportional guidance with over gravity compensation trajectory simulation platform, the effects of different factors on the high frequency jamming were evaluated. The result shows that interference frequency and wave gate width have a greater influence on the jamming effect, and the higher the frequency and the wider the wave gate are, the better the jamming effect is; the anti-interference performance of long cycle code is better than short cycle code; when the interference frequency reaches 100 kHz and wave door width reaches 20 μs, two space code can be completely jammed and miss distance can reach 510.4 m; jamming moment has a smaller effect on jamming effect. The results may provide some reference and basis for the development and application of high repetition jamming equipment.