A portable non-mydriatic fundus camera with 40° field of view is designed, and it can be used to capture retinal images. The optical system of this camera consists of lighting system and imaging system, in which the lighting system is equipped with visible light source and infrared focusing source. The use of infrared light source focusing can avoid the use of drugs on eye mydriatic treatment. The light source uses a unique circular LED and it is imaged on the human eye pupil to avoid reflection effect as it falls on the cornea. Focusing lens is placed in the imaging system, which has ±15D diopter compensation function. In imaging system, the root mean square of spot diagrams is less than 2.8 μm, the field curvature is less than 0.2 mm, the distortion is less than 0.6%, and the modulation transfer function values are higher than 0.4 at 70 lp/mm in all fields. The original image is clearly reproduced on the image plane by using Zemax software for imaging simulation. This camera can be used for the prevention and rehabilitation of disease in ophthalmic medical industry.