• Photonics Research
  • Vol. 8, Issue 6, 929 (2020)
Xueli Chen1、2、†,*, Xinyu Wang1、2、†, Lin Wang3、†, Peng Lin4, Yonghua Zhan1、2, and Ji-Xin Cheng4
Author Affiliations
  • 1Engineering Research Center of Molecular & Neuro Imaging, Ministry of Education, Xi’an 710126, China
  • 2School of Life Science and Technology, Xidian University, Xi’an 710126, China
  • 3School of Information Sciences and Technology, Northwest University, Xi’an 710127, China
  • 4Department of Electrical and Computer Engineering & Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA
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    DOI: 10.1364/PRJ.384604 Cite this Article Set citation alerts
    Xueli Chen, Xinyu Wang, Lin Wang, Peng Lin, Yonghua Zhan, Ji-Xin Cheng. Stimulated Raman scattering signal generation in a scattering medium using self-reconstructing Bessel beams[J]. Photonics Research, 2020, 8(6): 929 Copy Citation Text show less

    Abstract

    Scattering is a huge challenge for microscopic imaging. Indeed, it is difficult to observe target chemicals in scattering media by means of the current Gaussian beam-based stimulated Raman scattering (SRS) microscopy, since the tight focus of the Gaussian beam is destroyed after propagating through a certain distance. Bessel beams, featuring self-reconstructing property, may bring a solution to this problem. By combining Bessel beams with SRS microscopy, we can probe the SRS signal from a scattering medium. In this paper, using the beam propagation method, we first simulate the propagation of the Bessel beam as well as the generation and self-reconstruction of SRS signals. By adding glass beads along the beam propagation path in order to simulate scattering, the propagation of the Bessel beams and the generation of the SRS signals will change. Then, we design a series of simulations to investigate the influence of the size, position, number, and distribution of the added glass beads on the generation of the SRS signals. A preliminary experiment is also carried out to confirm the simulation predictions. Results demonstrate that the SRS signals can be generated or be recovered at a certain depth in scattering media, and that such signals are greatly affected by the parameters of the scatters.
    EB(x,y,z)=E011+(zzfzB)2e(rcw0)2[11+(zzfzB)2]J0(k0x2+y2sinα)eik0(zzf)cosα,(1)

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    E1(kx,ky,z+Δz/2)=F[EB(x,y,z)]eikx2+ky2k0n0Δz2,(2)

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    E2(x,y,z+Δz/2)=F1[E1(kx,ky,z+Δz/2)]eik0ΔnΔz,(3)

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    EBP(x,y,z+Δz)=F1{F[E2(x,y,z+Δz/2)]}eikx2+ky2k0n0Δz2,(4)

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    Δn=δ(x,y,z)|nsn0|,(5)

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    δ(x,y,z)={1(xxb)2+(yyb)2+(zzb)2R20(xxb)2+(yyb)2+(zzb)2>R2,(6)

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    IbSRS(x,y,z)=CIm(χ(3))|EBPP(x,y,z)|2|EBPS(x,y,z)|2Δz.(7)

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    Xueli Chen, Xinyu Wang, Lin Wang, Peng Lin, Yonghua Zhan, Ji-Xin Cheng. Stimulated Raman scattering signal generation in a scattering medium using self-reconstructing Bessel beams[J]. Photonics Research, 2020, 8(6): 929
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