In this paper, the sound-light fusion effect has been studied based on physiological parameters and subjective emotional changes. The influence of western major music, western minor music, 3 kinds of monochromatic lighting (wavelengths of 623, 537, 445 nm) and 2 kinds of white lighting with different color temperatures (color temperature of 3000, 6000 K) on the human body is systematically discussed. The experimental results show that people's physiological response to music is stronger than that of the lighting stimulus. The physiological perception under dual-factor stimulation is stronger than that of single-factor, and sound plays a major role in the sound-light fusion environment. Music and light interact when they stimulate emotions at the same time. The superposition of two positively related emotional environmental factors can strengthen the effect on emotions. It provides theoretical support and opinions for the design of music and light show or immersive interactive lighting.