A flat-field correction method is proposed to eliminate the interference fringes on the retinal photoreceptor images which origin from the etaloning effect in the thinned back illuminated CCD. First of all, the generation mechanism of etaloning effect is analyzed. Different from other flat-field method which uses the image of white balance reflectance target as reference image, the retinal image without adaptive optics compensation is used as reference image for flat-field correction method in this experiment, and the calibration result using the new reference image has a significant improvement compared with the result calibrated using the white balance reflectance target as reference image. As a means to quantify the calibrated result of flat-field method, the average power spectra of the photoreceptor image is calculated. The mean value of the average power spectra for the image after calibration is improvedly 132.1% compared with the image before calibration in the range of spatial frequency 70 and 90 lp/(°). Experimental result indicates that the image of retinal photoreceptor has a significant improvement using flat-field correction method.