Major factors that influence the performance parameters of high-speed charge coupled device (CCD), such as CCD’s frequency response characteristics, grounding return, electromagnetic interference, image cross talk et al., were analyzed and considerated. A high-speed CCD sensor with 400 pixel×400 pixel was designed, fabricated and tested. The analysis shows that the CCD sensor has good performances and features such as good responsibility, high dynamic range and low noise et al., It was greatly improved for the multi-tapped high-speed CCD sensor to optimize its uniformity among multi-tapped outputs and to reduce its noise and signal cross talk, which shows that the design and layout used are reasonable and efficient.