Jun WANG, Zhou-li WANG, Jing-jing CHENG. Interaction Between Tartrazine and Bovine Serum Albumin Using Multispectral Method and Molecular Docking[J]. Spectroscopy and Spectral Analysis, 2022, 42(3): 904
- Spectroscopy and Spectral Analysis
- Vol. 42, Issue 3, 904 (2022)
Fig. 1. Fluorescence spectra of tartrazine-BSA (293 K) c (BSA)=5×10-7 mol·L-1; c (tartrazine)(from up to down)=(0, 1, 2, 3, 4, 5)×10-7 mol·L-1
Fig. 2. Stern-Volmer curve for the fluorescence quenching of BSA by tartrazine
![lg[(F0-F)/F]-lg[Q] curve at different temperatures](/Images/icon/loading.gif){kind=icon}
Fig. 3. lg[(F 0-F )/F ]-lg[Q ] curve at different temperatures
Fig. 4. Fluorescence spectra of BSA and UV spectra of tartrazine c (BSA)=5×10-7 mol·L-1; c (tartrazine)=5×10-7 mol·L-1
Fig. 5. Synchronous fluorescence spectra of tartrazine and BSA c (BSA)=5×10-7 mol·L-1; c (tartrazine)(from up to down)=(0, 1, 2, 3, 4, 5)×10-7 mol·L-1
(a): Δλ =15 nm; (b): Δλ =60 nm
(a): Δ
Fig. 6. Three dimensional fluorescence spectra of BSA and tartrazine-BSA
(a):c (BSA)=5×10-7 mol·L-1; (b): c (BSA)=5×10-7 mol·L-1; c (tartrazine)=5×10-7 mol·L-1
(a):
Fig. 7. UV spectra of tartrazine and BSA c (BSA)=5×10-7 mol·L-1; c (tartrazine)(from down to up)=(0, 1, 2, 3, 4, 5)×10-7 mol·L-1
Fig. 8. Structure of BSA and tartrazine
Fig. 9. Molecular docking simulation of tartrazine and BSA
(a): Molecular docking of tartrazine and BSA;(b): Amino acid residues around tartrazine
(a): Molecular docking of tartrazine and BSA;(b): Amino acid residues around tartrazine
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Table 1. Stern-Volmer linear equations and correlation coefficients
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Table 2. Binding constant K A at different temperatures and number of binding point
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Table 3. Thermodynamic parameters of tartrazine-BSA interaction at different temperatures
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Table 4. Characteristic parameters of three-dimensional fluorescence spectra
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