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Advanced Photonics Nexus
Vol. 2, Issue 1, 016009 (2023)

Confocal rescan structured illumination microscopy for real-time deep tissue imaging with superresolution

Shuhao Shen^1、2, E Du^1、3, Miao Zhang¹, Yuting Wen¹, Kai Long¹, Anqi Qiu^1、4, and Nanguang Chen^1、4、*

Author Affiliations

¹National University of Singapore, Department of Biomedical Engineering, Singapore

²Xidian University, Guangzhou Institute of Technology, Guangzhou, China

³Shenzhen Institute of Information Technology, School of Microelectronics, Shenzhen, China

⁴National University of Singapore (Suzhou) Research Institute, Suzhou, China

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DOI: 10.1117/1.APN.2.1.016009 Cite this Article Set citation alerts

Shuhao Shen, E Du, Miao Zhang, Yuting Wen, Kai Long, Anqi Qiu, Nanguang Chen. Confocal rescan structured illumination microscopy for real-time deep tissue imaging with superresolution[J]. Advanced Photonics Nexus, 2023, 2(1): 016009 Copy Citation Text

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References

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[2] J.-A. Conchello, J. W. Lichtman. Optical sectioning microscopy. Nat. Methods, 2, 920-931(2005).

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[7] S. Pant et al. Line-scan focal modulation microscopy. J. Biomed. Opt., 22, 050502(2017).

[8] M. A. Neil, R. Juškaitis, T. Wilson. Method of obtaining optical sectioning by using structured light in a conventional microscope. Opt. Lett., 22, 1905-1907(1997).

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[11] J. Mertz. Optical sectioning microscopy with planar or structured illumination. Nat. Methods, 8, 811-819(2011).

[12] D. Lim et al. Optically sectioned in vivo imaging with speckle illumination HiLo microscopy. J. Biomed. Opt., 16, 016014(2011).

[13] X. Zhou et al. Double-exposure optical sectioning structured illumination microscopy based on Hilbert transform reconstruction. PLoS One, 10, e0120892(2015).

[14] M. G. Gustafsson, D. A. Agard, J. W. Sedat. Doubling the lateral resolution of wide-field fluorescence microscopy using structured illumination. Proc. SPIE, 3919, 141-150(2000).

[15] W. Qiao et al. Single-scan HiLo with line-illumination strategy for optical section imaging of thick tissues. Biomed. Opt. Express, 12, 2373-2383(2021).

[16] G. M. De Luca et al. Re-scan confocal microscopy: scanning twice for better resolution. Biomed. Opt. Express, 4, 2644-2656(2013).

[17] P. Sarder, A. Nehorai. Deconvolution methods for 3-D fluorescence microscopy images. IEEE Signal Process Mag., 23, 32-45(2006).

[18] A. Lal, C. Shan, P. Xi. Structured illumination microscopy image reconstruction algorithm. IEEE J. Sel. Top. Quantum Electron., 22, 50-63(2016).

[19] R. Cao et al. Inverse matrix based phase estimation algorithm for structured illumination microscopy. Biomed. Opt. Express, 9, 5037-5051(2018).

[20] N. T. Urban et al. STED nanoscopy of actin dynamics in synapses deep inside living brain slices. Biophys. J., 101, 1277-1284(2011).

[21] M. G. M. Velasco et al. 3D super-resolution deep-tissue imaging in living mice. Optica, 8, 442-450(2021).

[22] U. V. Nägerl et al. Live-cell imaging of dendritic spines by STED microscopy. Proc. Natl. Acad. Sci. U. S. A., 105, 18982-18987(2008).

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Shuhao Shen, E Du, Miao Zhang, Yuting Wen, Kai Long, Anqi Qiu, Nanguang Chen. Confocal rescan structured illumination microscopy for real-time deep tissue imaging with superresolution[J]. Advanced Photonics Nexus, 2023, 2(1): 016009

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