For the phase-sensitive signal of the total reflection prism laser gyros (TRPLG) is subjected to noise interference phenomenon during in frequency stabilization, a novel technique for the adaptive frequency stabilization of TRPLG is studied. The theory of frequency stabilization characteristic of the TRPLG is analyzed, combined with the principle of adaptive noise cancellation, a mathematic model of the adaptive frequency stabilization system in TRPLG is proposed. The control system based on recursive least square method for the adaptive frequency stabilization of TRPLG is built by the hardware circuit design. The experimental tests for the original frequency stabilization technique and adaptive frequency stabilization technique are conducted. The results show that, using adaptive frequency stabilization technique, the performance of phase-sensitive signal subjected to noise interference could be eliminated effectively and frequency stabilization accuracy is improved by nearly an order of magnitude, the gyros accuracy could be improved by over 60% . The analytic study provides an important reference for improving the performance of TRPLG.