• Journal of Semiconductors
  • Vol. 44, Issue 2, 023102 (2023)
Jiahao Zheng1, Chunyan Feng1、2, Songyin Qiu1, Ke Xu3, Caixia Wang1, Xiaofei Liu1, Jizhou Lv2, Haoyang Yu1, and Shaoqiang Wu1、*
Author Affiliations
  • 1Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing100176, China
  • 2Chinese Academy of Inspection and Quarantine Center for Biosafety, Sanya 572024, China
  • 3National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
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    DOI: 10.1088/1674-4926/44/2/023102 Cite this Article
    Jiahao Zheng, Chunyan Feng, Songyin Qiu, Ke Xu, Caixia Wang, Xiaofei Liu, Jizhou Lv, Haoyang Yu, Shaoqiang Wu. Application and prospect of semiconductor biosensors in detection of viral zoonoses[J]. Journal of Semiconductors, 2023, 44(2): 023102 Copy Citation Text show less
    (Color online) Schematic process of the detection of biosensor. Samples are collected from animals, human, and environment, then the virus particles, antibodies, RNA or DNA are targeted and detected by semiconductor biosensors. The signals are finally displayed by visible image.
    Fig. 1. (Color online) Schematic process of the detection of biosensor. Samples are collected from animals, human, and environment, then the virus particles, antibodies, RNA or DNA are targeted and detected by semiconductor biosensors. The signals are finally displayed by visible image.
    (Color online) Schematic representation of the major principles of semiconductor biosensors. (a) Schematic representation of the identification process, the key point is the recognition and conversion element. (b) Schematic representation of the major principles for optical biosensors. (c) Schematic representation of the major principles for electrochemical biosensors.
    Fig. 2. (Color online) Schematic representation of the major principles of semiconductor biosensors. (a) Schematic representation of the identification process, the key point is the recognition and conversion element. (b) Schematic representation of the major principles for optical biosensors. (c) Schematic representation of the major principles for electrochemical biosensors.
    (Color online) Schematic representation of the major principles of graphene based semiconductor biosensors. (a) Representation of the major principles of graphene FET biosensors. (b) Representation of the major principles of graphene based optical biosensors.
    Fig. 3. (Color online) Schematic representation of the major principles of graphene based semiconductor biosensors. (a) Representation of the major principles of graphene FET biosensors. (b) Representation of the major principles of graphene based optical biosensors.
    (Color online) Schematic representation of the major principle of silicon nanowire based semiconductor biosensor.
    Fig. 4. (Color online) Schematic representation of the major principle of silicon nanowire based semiconductor biosensor.
    AnalyteMethodDetection targetLimit of detectionRef.
    Hepatitis B virusSilicon nanowire field-effect transistorHBsAg and HBx3.92 fM–0.39 pM; 5.61 fM–0.56 pM[56]
    Hepatitis B virusPolycrystalline silicon NWFET sensorsHBsAg4.02 × 10−18 g/mL[57]
    Influenza virusSilicon nanowire field-effect transistorHemagglutinin1 fM HA[58]
    COVID-19Silicon nanowire field-effect transistorsSpike proteinNot mentioned[59]
    SARS-CoV-2Silicon nanowire arraysSpike protein100 ng/mL (or 575 pM)[60,61]
    Table 0. Summary of Silicon Nanowire-based biosensors which applied in detection of viral zoonoses.
    Strategies to amplify the aimed nucleotidesConstant/variable temperatureTypical detection zoonosesRef.
    Rolling circle amplification (RCA)Constant temperatureEbola[21]
    Polymerase chain reaction (PCR)Variable temperatureHBV[24]
    Hybridization chainreaction (HCR)Constant temperatureInfluenza (H1N1)[23]
    Loop-mediated isothermal amplification (LAMP)Constant temperatureDengue[22]
    Recombinase polymerase amplification (RPA)Constant temperatureCOVID-19[25]
    Table 0. Amplification strategies used to amplify the aimed nucleotides of the pathogens of viral zoonoses.
    VirusMethodMaterialLimit of detectionRef.
    Inflenza virus (H1N1)OpticalG/Au-Metal oxide complex7.27 fg/mL[39]
    Inflenza virus (H1N1)ElectrorGO/AuNPs10−8 U/mL[40]
    Inflenza virus (H1N1)ElectrorGO33 PFU/mL[41]
    Inflenza virus (H1N1)OpticalrGO3.8 pg/mL[42]
    Ebola virusElectrorGO2.4 pg/mL[43]
    Ebola virusElectrorGO1μg/mL[44]
    Dengue virusOpticalCdSQDs-NH2-GO1 pM[45]
    Dengue virusOpticalrGO0.08 pM[46]
    Dengue virusOptical/ElectroGO/Ru0.38 ng/mL[47]
    Dengue virusOpticalrGO/PAMAM0.08 pM[45,46]
    Hepatitis C virusOpticalrGO10 fM[48]
    Hepatitis C virusOpticalrGO/CuNPs0.4 nM[49]
    Hepatitis C virusElectroGO0.2 nM[50]
    SARS-COV-2ElectroG1.6 PFU/mL[51]
    ZIKA virusElectroG/CVD0.5 nM[52]
    HIVElectroG/CVD0.1 ng/mL[53]
    HIVElectroGO/PANi100 aM[54]
    Hepatitis B virusElectrorGO/AuNPs3.8 ng/mL[55]
    Hepatitis B virusElectroGQDs1 nM[56]
    Table 0. The overview of the graphene-based biosensors detection of viral zoonoses in the last 5 years. (Table was complemented based on Ref. [38]).
    AnalyteMethodLimit of detectionDetection rangeRef.
    Dengue virus NS1 proteinAmperometry35 000 pg/mL1000–2500 ng/mL[32]
    Hepatitis B virus genomic DNADifferential pulsevoltammetry3.5 fM10−14–10−8 ng/mL[33]
    SARS-CoV-2 spike proteinFluorescence35 mg/LNot mentioned[34]
    Dengue virus (whole)Chemiresistive8.4 × 102 TCID50/mL8.4 × 102–8.4 × 105 TCID50/mL[35]
    Avian influenza virus (H5N1) DNA sequenceChemiresistiveNR2–200 pM[36]
    SARS-CoV-2 spike proteinTransistor0.55 fg/mL0.0055–5.5 pg/mL[37]
    SARS-CoV-2 nucleocapsid proteinTransistor0.016 fg/mL0.016–16 pg/mL[37]
    Table 0. Summary of CNT based biosensors which applied in detection of viral zoonoses.
    Jiahao Zheng, Chunyan Feng, Songyin Qiu, Ke Xu, Caixia Wang, Xiaofei Liu, Jizhou Lv, Haoyang Yu, Shaoqiang Wu. Application and prospect of semiconductor biosensors in detection of viral zoonoses[J]. Journal of Semiconductors, 2023, 44(2): 023102
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