Structure-Guided Development of Reversibly Photoswitchable Green Fluorescent Proteins Probe

Wang Sheng; Chen Xuanze; Chang Lei; Xue Ruiying; Sun Yujie

doi:10.3788/aos201737.0318002

[1] Heim R, Prasher D C, Tsien R Y. Wavelength mutations and posttranslational autoxidation of green fluorescent protein［C］. Proc Natl Acad Sci U S A, 1994, 91(26): 12501-12504.

[2] Shaner N C, Campbell R E, Steinbach PA, et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein［J］. Nat Biotechnol, 2004, 22(12): 1567-1572.

[3] Shcherbo D, Merzlyak E M, Chepurnykh T V, et al. Bright far-red fluorescent protein for whole-body imaging［J］. Nat Methods, 2007, 4(9): 741-746.

[4] Adam V, Berardozzi R, Byrdin M, et al. Phototransformable fluorescent proteins: future challenges［J］. Current Opinion in Chemical Biology, 2014, 20: 92-102.

[5] Adam V. Phototransformable fluorescent proteins: which one for which application［J］. Histochemistry and Cell Biology, 2014, 142(1): 19-41.

[6] Post J N, Lidke K A, Rieger B, et al. One- and two-photon photoactivation of a paGFP-fusion protein in live Drosophila embryos［J］. FEBS Lett, 2005, 579(2): 325-330.

[7] Mutoh T, Miyata T, Kashiwagi S, et al. Dynamic behavior of individual cells in developing organotypic brain slices revealed by the photoconvertable protein Kaede［J］. Exp Neurol, 2006, 200(2): 430-437.

[8] Habuchi S, Ando R, Dedecker P, et al. Reversible single-molecule photoswitching in the GFP-like fluorescent protein Dronpa［C］. Proc Natl Acad Sci U S A, 2005, 102(27): 9511-9516.

[9] Jensen N A, Danzl J G, Willig K I, et al. Coordinate-targeted and coordinate-stochastic super-resolution microscopy with the reversibly switchable fluorescent protein Dreiklang［J］. Chemphyschem, 2014, 15(4): 756-762.

[10] Zhang X, Zhang M, Li D, et al. Highly photostable, reversibly photoswitchable fluorescent protein with high contrast ratio for live-cell superresolution microscopy［C］. Proc Natl Acad Sci U S A, 2016, 113(37): 10364-10369.

[11] Wang S, Chen X Z, Chang L, et al. GMars-Q enables long-term live-cell parallelized reversible saturable optical fluorescence transitions nanoscopy［J］. ACS Nano, 2016, 10(10): 9136-9144.

[12] Bourgeois D, Adam V. Reversible photoswitching in fluorescent proteins: a mechanistic view［J］. IUBMB Life, 2012, 64(6): 482-491.

[13] Creemers T M, Lock A J, Subramaniam V V, et al. Three photoconvertible forms of green fluorescent protein identified by spectral hole-burning［J］. Nature Structural Biology, 1999, 6(7): 557-560.

[14] Bizzarri R, Serresi M, Cardarelli F, et al. Single amino acid replacement makes Aequorea victoria fluorescent proteins reversibly photoswitchable［J］. J Am Chem Soc, 2010, 132(1): 85-95.

[15] Grotjohann T, Testa I, Leutenegger M, et al. Diffraction-unlimited all-optical imaging and writing with a photochromic GFP［J］. Nature, 2011, 478(7368): 204-208.

[16] Grotjohann T, Testa I, Reuss M, et al. rsEGFP2 enables fast RESOLFT nanoscopy of living cells［J］. eLife, 2012, 1: e00248.

[17] Chmyrov A, Keller J, Grotjohann T, et al. Nanoscopy with more than 100,000′ doughnuts′［J］. Nat Methods, 2013, 10(8): 737-740.

[18] Duwé S, de Zitter E, Gielen V, et al. Expression-enhanced fluorescent proteins based on enhanced green fluorescent protein for super-resolution microscopy［J］. ACS Nano, 2015, 9(10): 9528-9541.

[19] Khatib M E, Martins A, Bourgeois D, et al. Rational design of ultrastable and reversibly photoswitchable fluorescent proteins for super-resolution imaging of the bacterial periplasm［J］. Sci Rep, 2016, 6: 18459.

[20] Brakemann T, Stiel A C, Weber G, et al. A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching［J］. Nat Biotechnol, 2011, 29(10): 942-947.

[21] Andresen M, Stiel A C, Trowitzsch S, et al. Structural basis for reversible photoswitching in Dronpa［C］. Proc Natl Acad Sci U S A, 2007, 104(32): 13005-13009.

[22] Stiel A C, Trowitzsch S, Weber G, et al. 1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants［J］. Biochem J, 2007, 402(1): 35-42.

[23] Adam V, Moeyaert B, David C C, et al. Rational design of photoconvertible and biphotochromic fluorescent proteins for advanced microscopy applications［J］. Chem Biol, 2011, 18(10): 1241-1251.

[24] Chang H, Zhang M, Ji W, et al. A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications［C］. Proc Natl Acad Sci U S A, 2012, 109(12): 4455-4460.

[25] Zhang X, Chen X, Zeng Z, et al. Development of a reversibly switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI)［J］. ACS Nano, 2015, 9(3): 2659-2667.

[26] Dertinger T, Colyer R, Vogel R, et al. Superresolution optical fluctuation imaging (SOFI)［J］. Adv Exp Med Biol, 2011, 733: 17-21.

[27] Wang S, Moffitt J R, Dempsey G T, et al. Characterization and development of photoactivatable fluorescent proteins for single-molecule-based superresolution imaging［C］. Proc Natl Acad Sci U S A, 2014, 111(23): 8452-8457.

[28] Nienhaus K, Nienhaus G U. Photoswitchable fluorescent proteins: do not always look on the bright side［J］. ACS Nano, 2016, 10(10): 9104-9108.

[29] Andresen M, Stiel A C, Folling J, et al. Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy［J］. Nat Biotechnol, 2008, 26(9): 1035-1040.

[30] Tiwari D K, Arai Y, Yamanaka M, et al. A fast-and positively photoswitchable fluorescent protein for ultralow-laser-power RESOLFT nanoscopy［J］. Nat Methods, 2015, 12(6): 515-518.

[31] Shcherbakova D M, Sengupta P, Lippincott-Schwartz J, et al. Photocontrollable fluorescent proteins for superresolution imaging［J］. Annual Review of Biophysics, 2014, 43: 303-329.

[32] Stiel A C, Andresen M, Bock H, et al. Generation of monomeric reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy［J］. Biophys J, 2008, 95(6): 2989-2997.

[33] Lavoie-Cardinal F, Jensen N A, Westphal V, et al. Two-color RESOLFT nanoscopy with green and red fluorescent photochromic proteins［J］. Chemphyschem, 2014, 15(4): 655-663.

[34] Pakhomov A A, Martynov V I. GFP family: structural insights into spectral tuning［J］. Chemistry Biology, 2008, 15(8): 755-764.