In recent years, people pay more and more attentions to the development of reversibly photoswitchable fluorescent proteins (RSFPs), because those fluorescent proteins can greatly promote the development and application of live-cell super-resolution imaging techniques. Reversibly photoswitchable fluorescent protein can be repeatedly and reversibly modulated by different wavelength lights, thus it can be widely applied in many fields such as high density data optical storage, the measurement of photochromic fluorescence resonance energy transfer, and super-resolution imaging based on reversible saturable linear fluorescence transitions (RESOLFT) principle. The recent development of green RSFPs is reviewed from the key amino acids involved in RSFPs design. We also make a brief discussion of the relationship between structure and optical characteristics of RSFPs, serving as the reference for further structure-guided development of better RSFPs.