The flurorescecne spectra of lycopene in different solvents including hexane, ethyl acetate, terrahydrofuran, chloroform, acetone, benzene and of lycopene-terrahydrofuran solution with different concentrations are measured by a fluorescence spectrophotometer. It is found that the wavelength of fluorescence spectra maximum (λmax) change along with the variation of the polarity of solvent. The transition energies (ET) of lycopene calculated in different solvents are 220.5 kJ/mol, 218.2 kJ/mol, 215.6 kJ/mol, 215.2 kJ/mol, 214.9 kJ/mol, 211.5 kJ/mol. When the concentration of lycopene-terrahydrofuran solution is lower than 50 μg/ml, the intensity of fluorescence increases with the increase of solution concentration, when the concentration is higher than 50 μg/ml, the intensity of fluorescence decreases with the increase of the concentration, which is caused by the interaction between molecules in the excited state and the ground state. Three weak peaks appear in the fluorescence spectra of solutions when the concentration of which is lower than 80 μg/ml. The corresponding transition energies can be calculated as below: E(1)T=278.2 kJ/mol、E(2)T=260.2 kJ/mol and E(3)T=239.3 kJ/mol.