Cell biology has come into a new era by the hand of super-resolution microscopy. However, how to improve the temporal-spatial resolution of super-resolution microscopy is still an important problem to be resolved in the field of optics. So far, almost all of the super-resolution microscopies are depend on fluorescent probes. As a unique series of fluorescent probes, light regulated fluorescent proteins can be activated by excitation light of different wavelengths to produce stochastic or specially patterned signals. Using this information, the spatial resolution of lens system is improved. By summarizing different parameters of light regulated fluorescent proteins, we focus on fluorescent probes to discuss the way of improving spatial resolution of imaging system. It provides advices to choose proper fluorescent probes, and the relationship between fluorescent proteins and super-resolution microscopy is expounded.