1064 nm, 532 nm frequency-doubled antireflection coating was fabricated by using electron-beam evaporation on LiB3O5 （LBO）. The optical property, adhesion and laser-induced damage threshold were determined by Lambda 900 spectrometer, MTS Nano Indenter and Q-switched pulse laser, respectively. The results showed that 1064 nm, 532 nm frequency-doubled antireflection coating with good performances are obtained by improving deposition parameters. The reflectance of the coating at wavelength of 1064 nm and 532 nm are 0.07% and 0.16%. The critical adhesion and the laser-induced damage threshold are 137.4 mN and 15.14 J/cm2. It is found that the damage of the coating locates in the layer of Al2O3.