The deactivation rate constants by 6 different collision partners of vibrationally excited C6F6 have been measured for the first time using an IR-UV double resonance technique, for example, KO2= (8.8±0.4) ×103 sec-1.Torr-1, KCO22= (7.1 ±0.8) ×103 sec-1 · Torr-1 and KXe = (2.9± 0.47) × 104 sec-1 · Torr-1. The measured value of KC6F6 = (0.4±0.15)×106seo-1·Torr-1 agrees well with the Lambert-Salter empirical correlation.