One-photon persistent spectral hole-burning was reported for the organic guest molecule 5, 10, 15, 20-tetra-(p-hydroxyphenyl) prorphin (THP) doped into a poly (methylmethaorylate) (PMMA) amorphous matrix at liquid helium temperature. Hole properties and conditions for hole formation were investigated. Laser induced tautomerization was believed responsible for the hole burning. Stability of the holes was demonstrated longer than hours. Hole erasing and repeatable hole burning were realized.