• Laser & Optoelectronics Progress
  • Vol. 58, Issue 3, 3000041 (2021)
Chenyang Lü and Zhan Renjun*
Author Affiliations
  • College of Equipment Management and Support, Engineering University of People''s Armed Police, Xi''an , Shaanxi 710086, China
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    DOI: 10.3788/LOP202158.0300004 Cite this Article Set citation alerts
    Chenyang Lü, Zhan Renjun. Measurement Method of Optical Property Parameters of Biological Tissue[J]. Laser & Optoelectronics Progress, 2021, 58(3): 3000041 Copy Citation Text show less
    Schematic of experimental setup[21]
    Fig. 1. Schematic of experimental setup [21]
    Schematic of DIS system[34]
    Fig. 2. Schematic of DIS system [34]
    Kubelka-Munk theory flux
    Fig. 3. Kubelka-Munk theory flux
    Schematic of IAD/IS system[103]
    Fig. 4. Schematic of IAD/IS system [103]
    IAD/IMC system logic diagram[120]
    Fig. 5. IAD/IMC system logic diagram [120]
    Relationship of optical parameter measurement methods of biological tissue
    Fig. 6. Relationship of optical parameter measurement methods of biological tissue
    ClassificationAdvantageLimitation
    SIS

    ① No need to measure reflectivity of inner wall

    ② Two integrating spheres have no influence on each other

    ③ Calibration of system is easier and experimental device is simpler

    ④ Lower cost

    ① Low measurement accuracy

    ② Can only be measured in steps

    ③ Sample cannot be illuminated diffusely in transmission geometry

    DIS

    ① Most accurate method at present

    ② Suitable for layered structure organization

    ③ Diffuse reflectivity and diffuse transmittance can be measured at same time

    ④ Sample can be illuminated by diffuse reflection in transmission or reflection geometry

    ① Demand for stability of light source is very high

    ② It is difficult to implement and calibrate

    ③ Price is relatively high

    PSM① Measurement of absorption coefficient in Ultraviolet Band

    ① It is only suitable for in vitro measurement

    ② Scope of application is narrow

    CW① Information of organizational structure and function can be obtained at same time① Only large tissues and organs can be measured
    ② Limited to in vivo measurement
    Micro-area

    ① Measurement accuracy is higher

    ② Micro-areas can be measured

    ① Theory of photon transmission in micro-area is not accurate enough
    ② Limited to in vivo measurement
    Table 1. Classification and feature comparison of main measurement techniques
    ClassificationAdvantageLimitation
    Direct measurement①Analytical expression is very simple

    ①Experimental conditions specified in model must be strictly met

    ②Error is large for plane medium with multiple scattering

    Indirect measurement

    ①Can use computer-aided means

    ②High flexibility and accuracy

    ①Analytical method is complicated
    Table 2. Comparative analysis of different measurement methods
    ClassificationApplicable conditionAdvantageLimitation
    Non-iterative method

    ① Scattering is obviously stronger than absorption

    ② Relationship between optical parameters and measured parameters is an implicit function

    ① Mathematical form is simple

    ① It only aims at one-dimensional diffuse radiation that scattering far exceeds absorption.

    ② Theoretical model is not perfect

    Iterative method

    ① Samples with differences in refractive index at boundary

    ② Samples with multi-layer properties

    ① Theoretical model is more perfect

    ② Non-destructive measurement

    ① Using complex solution of transport equation
    Table 3. Applicable conditions and comparative analysis of indirect measurement method
    ClassificationApplicable scopeAdvantageLimitation
    IADSamples with prominent anisotropic scattering and internal boundary reflection

    ① Iterative solution can be obtained quickly with help of computer

    ② Anisotropic scattering and internal reflection at boundary can be flexibly included

    ③Result is very accurate when illumination diameter is less than sample diameter

    Solution can only be solved under conditions that tissue thickness is limited, optical parameters are uniformly distributed and solution process is independent of time
    IMCIt is most effective for tissues based on real shapes or anatomical structures

    ① Recognized as most accurate measurement method at present

    ② Loss of light at edge of sample can be accurately calculated

    Amount of calculation is large and calculation time is long
    Table 4. Application scope and comparative analysis of different iterative measurement methods
    Chenyang Lü, Zhan Renjun. Measurement Method of Optical Property Parameters of Biological Tissue[J]. Laser & Optoelectronics Progress, 2021, 58(3): 3000041
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