② Two integrating spheres have no influence on each other
③ Calibration of system is easier and experimental device is simpler
④ Lower cost
① Low measurement accuracy
② Can only be measured in steps
③ Sample cannot be illuminated diffusely in transmission geometry
DIS
① Most accurate method at present
② Suitable for layered structure organization
③ Diffuse reflectivity and diffuse transmittance can be measured at same time
④ Sample can be illuminated by diffuse reflection in transmission or reflection geometry
① Demand for stability of light source is very high
② It is difficult to implement and calibrate
③ Price is relatively high
PSM
① Measurement of absorption coefficient in Ultraviolet Band
① It is only suitable for in vitro measurement
② Scope of application is narrow
CW
① Information of organizational structure and function can be obtained at same time
① Only large tissues and organs can be measured
② Limited to in vivo measurement
Micro-area
① Measurement accuracy is higher
② Micro-areas can be measured
① Theory of photon transmission in micro-area is not accurate enough
② Limited to in vivo measurement
Table 1. Classification and feature comparison of main measurement techniques
Classification
Advantage
Limitation
Direct measurement
①Analytical expression is very simple
①Experimental conditions specified in model must be strictly met
②Error is large for plane medium with multiple scattering
Indirect measurement
①Can use computer-aided means
②High flexibility and accuracy
①Analytical method is complicated
Table 2. Comparative analysis of different measurement methods
Classification
Applicable condition
Advantage
Limitation
Non-iterative method
① Scattering is obviously stronger than absorption
② Relationship between optical parameters and measured parameters is an implicit function
① Mathematical form is simple
① It only aims at one-dimensional diffuse radiation that scattering far exceeds absorption.
② Theoretical model is not perfect
Iterative method
① Samples with differences in refractive index at boundary
② Samples with multi-layer properties
① Theoretical model is more perfect
② Non-destructive measurement
① Using complex solution of transport equation
Table 3. Applicable conditions and comparative analysis of indirect measurement method
Classification
Applicable scope
Advantage
Limitation
IAD
Samples with prominent anisotropic scattering and internal boundary reflection
① Iterative solution can be obtained quickly with help of computer
② Anisotropic scattering and internal reflection at boundary can be flexibly included
③Result is very accurate when illumination diameter is less than sample diameter
Solution can only be solved under conditions that tissue thickness is limited, optical parameters are uniformly distributed and solution process is independent of time
IMC
It is most effective for tissues based on real shapes or anatomical structures
① Recognized as most accurate measurement method at present
② Loss of light at edge of sample can be accurately calculated
Amount of calculation is large and calculation time is long
Table 4. Application scope and comparative analysis of different iterative measurement methods