In consideration of the problem of the large usage of random seeds in the current privacy amplification schemes, one design scheme of privacy amplification in quantum key distribution (QKD) based on modular and generalized Trevisan’s randomness extractor construction is proposed, and the confirmation of its security with the help of quantum side information analysis theory is presented. The results indicate that such a scheme not only resists against quantum attacks but also effectively reduces the usage of random seeds to ensure an efficient and malleable privacy amplification.