Excessive use of abamectin in agricultural production causes excessive crop pesticide residues. This study used a JASCO FP8300 fluorescence spectrophotometer to detect abamectin pesticide solution.The fluorescence spectrum characteristics of the abamectin technical solution and preparation solution were analyzed to provide a data reference basis for the rapid detection of abamectin. The experiment first analyzed the three-dimensional fluorescence spectra of the technical solution and two preparation solutions from different manufacturers. It is determined that the fluorescence characteristic peak area belonging to the abamectin is Ex=250~ 290 nm, Em=280~320 nm, and the best excitation wavelength is 270 nm. Therefore, 270 nm was selected as the best excitation band for two-dimensional fluorescence spectrum detection. According to the two-dimensional spectral data, the fluorescence intensity of abamectin fluorescence characteristic peak was analyzed with the change of solution concentration, and the prediction model of them was obtained by fitting relevant data. According to the results of data analysis, theR² of the prediction model of abamectin technical solution was 0.999, and the root means square error (RMSE) of the prediction results was 0.359 mg·L^-1 in the concentration range of 10~35 mg·L^-1. Besides, theR² of the abamectin preparation solution prediction model produced by two different manufacturers within the concentration range of 10~35 mg·L^-1 were 0.935 and 0.985. Moreover, the root means square error (RMSE) of the predicted results were 1.945 and 0.858 mg·L^-1. The results showed that the fluorescence effect of abamectin in the preparation could not be lost due to other fillers and additives. In addition, the concentration of abamectin could be reflected by the fluorescence intensity value, which further verified the feasibility of using the fluorescence spectrum to detect the content of abamectin.