Zhibing Xu, Wenxia Zhou, Dongdong Xu, Xiao Wang, Jianhua Yin, Huijie Wang. Research Progress of Fluorescence Polarization Modulation Microscopy Imaging Technology[J]. Laser & Optoelectronics Progress, 2021, 58(24): 2400006
- Laser & Optoelectronics Progress
- Vol. 58, Issue 24, 2400006 (2021)
Fig. 1. Absorption and radiative dipole moments of fluorescence dipole. (a) Dependence of fluorescence dipole absorption probability on angle between absorption dipole moment and electric field of exciting light; (b) emission intensity pattern of fluorescence dipole
Fig. 2. Schematic for measuring FA of molecules
Fig. 3. Schematic for measuring LD of molecules
Fig. 4. Geometrical models with different dipole orientation distributions obtained by TP-FPM method [26]
Fig. 5. Schematic of experiment setup of SPoD
Fig. 6. Images of hippocampal neurons (scale bar: 1 μm) [33]. (a) WF images; (b) SPoD images
Fig. 7. SDOM images of septin in live yeast cells (scale bar: 500 nm) [34]. (a) Orientation mapping images of septin with WF (upper-left) and SDOM (lower-right) under top view; (b) orientation mapping images under cross-sectional view
Fig. 8. Schematic of experiment setup of typical SIM
Fig. 9. pSIM imaging results of actin in BAPE cells (scale bar: 5 μm) [35]. (a) Intensity images by WF and SIM; (b) pseudocolored images in dipole direction by PM and pSIM; (c) magnified view of boxed region in Fig.9 (a)
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