Design of Large Field of View and High Numerical Aperture Microscope Objective

Muwang Huang; Xu Liu; Faguan Lin

doi:10.3788/LOP220458

Journals >Laser & Optoelectronics Progress >Volume 60 >Issue 5 >Page 0518001 > Article

Laser & Optoelectronics Progress
Vol. 60, Issue 5, 0518001 (2023)

Design of Large Field of View and High Numerical Aperture Microscope Objective

Muwang Huang^1、2, Xu Liu^1、*, and Faguan Lin²

Author Affiliations

¹College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, Zhejiang, China

²Foctek Photonics, Inc., Fuzhou 350100, Fujian, China

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DOI: 10.3788/LOP220458 Cite this Article Set citation alerts

Muwang Huang, Xu Liu, Faguan Lin. Design of Large Field of View and High Numerical Aperture Microscope Objective[J]. Laser & Optoelectronics Progress, 2023, 60(5): 0518001 Copy Citation Text

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Fig. 1. Initial system structure

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Fig. 2. Final design result

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Fig. 3. MTF curve

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Fig. 4. Wavefront tolerance analysis curve

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Parameter	Nanoscribe		Olympus	ZEISS	Our project
NA	1.4	0.8	1.3	1.3	1.3
Mag.	63×	25×	100×	100×	100×
W.D.	0.36	0.38	0.2	0.2	0.5
F.D.	0.28	0.7	0.265	0.23	1.00
C.G.	—	—	0.17	0.17	—
nd	1.515	1.515	1.515	1.515	1.522
vd	—	—	40.8	41.58	34.1

Table 1. Objective specification comparison

Parameter	Spec
Spectral range /nm	532-780
Objective field number	1.0
Numerical aperture	≥1.3
Working distance	≥0.5
Total length	<250
RMS of wavefront error /λ	0.09
Distortion /%	<0.5

Table 2. Objective design specs

Magnification	NA	Number of lens	NA·y	（NA）²·y
10×	0.22	4	0.176	0.039
20×	0.49	5	0.196	0.096
47.5×	0.91	7	0.153	0.139
98×	1.28	7	0.104	0.134

Table 3. Comparison of various microscope objectives

Parameter	Spec
Spectral range /nm	532-780
Effective focal length	7.5
Half field of view /（°）	4.2
Objective field number	1.0
Numerical aperture	1.316
Working distance	0.5
Total length	231.1
RMS of wavefront error /λ	0.05
Distortion /%	0.21
Number lens element	27

Table 4. Specs of designed objective

Item	Value
Airspce	0.005-0.01
Tilt /rad	0.00007-0.00029
Decenter	0.003-0.01

Table 5. Alignment tolerances

Item	Value
Defocus of image surface	0.001
Lens 13（X direction）	0.0105
Lens 13（Y direction）	0.0175
Lens 25（X direction）	0.0434
Lens 25（Y direction）	0.0505

Table 6. Compensator range

Muwang Huang, Xu Liu, Faguan Lin. Design of Large Field of View and High Numerical Aperture Microscope Objective[J]. Laser & Optoelectronics Progress, 2023, 60(5): 0518001

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