Fig. 1. The free amino group content of glycosylation of BSA treated with different concentrations of urea
Fig. 2. Fluorescence spectra of glycosylation of BSA treated with different concentrations of urea
a→b: 0, 1, 2, 3, 4, 5, 6, 7 mol·L-1
Fig. 3. The space structure of BSA
Fig. 4. Synchronous fluorescence spectra after glycosylation of BSA in different urea treatments
(a): Δλ=15 nm, (a→b): 1, 2, 3, 0, 4, 5, 6, 7 mol·L-1; (b): Δλ=60 nm, (a→b): 0, 1, 2, 3, 4, 5, 6, 7 mol·L-1
Fig. 5. Ultraviolet scanning spectra after glycosylation of BSA in different urea treatments
a→b: 3, 4, 5, 7, 6, 2, 1, 0 mol·L-1
Fig. 6. 3D fluorescence spectra after glycosylation of BSA and BSA in different urea treatments
Samples | Ex/Emmax/nm | a.u. |
---|
0 mol·L-1 | 280/375 | 1 116 | 1 mol·L-1 | 280/380 | 1 160 | 2 mol·L-1 | 280/380 | 1 212 | 3 mol·L-1 | 280/380 | 1 239 | 4 mol·L-1 | 280/380 | 1 243 | 5 mol·L-1 | 280/370 | 1 237 | 6 mol·L-1 | 280/370 | 1 197 | 7 mol·L-1 | 280/370 | 1 247 | 糖基化样品 | 280/370 | 1 334 |
|
Table 1. Typical three-dimensional fluorescence peaks