• Spectroscopy and Spectral Analysis
  • Vol. 41, Issue 2, 478 (2021)
Xue LI1、1, Li WANG1、1, Guang-xian LIU1、1, and Zong-cai TU1、1
Author Affiliations
  • 1[in Chinese]
  • 11. Institute of Agricultural Processing, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
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    DOI: 10.3964/j.issn.1000-0593(2021)02-0478-06 Cite this Article
    Xue LI, Li WANG, Guang-xian LIU, Zong-cai TU. Effect of Urea on Glycosylation of BSA Based on Spectral Techniques[J]. Spectroscopy and Spectral Analysis, 2021, 41(2): 478 Copy Citation Text show less
    The free amino group content of glycosylation of BSA treated with different concentrations of urea
    Fig. 1. The free amino group content of glycosylation of BSA treated with different concentrations of urea
    Fluorescence spectra of glycosylation of BSA treated with different concentrations of ureaa→b: 0, 1, 2, 3, 4, 5, 6, 7 mol·L-1
    Fig. 2. Fluorescence spectra of glycosylation of BSA treated with different concentrations of urea
    ab: 0, 1, 2, 3, 4, 5, 6, 7 mol·L-1
    The space structure of BSA
    Fig. 3. The space structure of BSA
    Synchronous fluorescence spectra after glycosylation of BSA in different urea treatments(a): Δλ=15 nm, (a→b): 1, 2, 3, 0, 4, 5, 6, 7 mol·L-1; (b): Δλ=60 nm, (a→b): 0, 1, 2, 3, 4, 5, 6, 7 mol·L-1
    Fig. 4. Synchronous fluorescence spectra after glycosylation of BSA in different urea treatments
    (a): Δλ=15 nm, (ab): 1, 2, 3, 0, 4, 5, 6, 7 mol·L-1; (b): Δλ=60 nm, (ab): 0, 1, 2, 3, 4, 5, 6, 7 mol·L-1
    Ultraviolet scanning spectra after glycosylation of BSA in different urea treatmentsa→b: 3, 4, 5, 7, 6, 2, 1, 0 mol·L-1
    Fig. 5. Ultraviolet scanning spectra after glycosylation of BSA in different urea treatments
    ab: 3, 4, 5, 7, 6, 2, 1, 0 mol·L-1
    3D fluorescence spectra after glycosylation of BSA and BSA in different urea treatments
    Fig. 6. 3D fluorescence spectra after glycosylation of BSA and BSA in different urea treatments
    SamplesEx/Emmax/nma.u.
    0 mol·L-1280/3751 116
    1 mol·L-1280/3801 160
    2 mol·L-1280/3801 212
    3 mol·L-1280/3801 239
    4 mol·L-1280/3801 243
    5 mol·L-1280/3701 237
    6 mol·L-1280/3701 197
    7 mol·L-1280/3701 247
    糖基化样品280/3701 334
    Table 1. Typical three-dimensional fluorescence peaks
    Xue LI, Li WANG, Guang-xian LIU, Zong-cai TU. Effect of Urea on Glycosylation of BSA Based on Spectral Techniques[J]. Spectroscopy and Spectral Analysis, 2021, 41(2): 478
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