Xian-qing WANG, Tong WEI, Yong YANG, Yan-guo SHI. Molecular Structure of Two Glutamate Decarboxylases From Mung Bean [Vigna Radiate (L.)] Analyzed by Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2020, 40(12): 3953
- Spectroscopy and Spectral Analysis
- Vol. 40, Issue 12, 3953 (2020)
Fig. 1. Purification of mung bean’s GAD by DEAE-Sepharose FF chromatography (a) and Superdex 200 chromatography (b)
Fig. 2. Purification of mung bean’s GAD by Superdex 200 chromatography
(a): GAD1; (b): GAD2
(a): GAD1; (b): GAD2
Fig. 3. HPLC separation of GAD1 and GAD2 from Mung Bean
Fig. 4. Subunit composition of mung bean GAD
Fig. 5. The optimum pH of pH stability of GAD activity
(a): Optimum pH of GAD activity;(b): pH stability of GAD activity
(a): Optimum pH of GAD activity;(b): pH stability of GAD activity
Fig. 6. The optimum temperature of temperature stability of GAD activity
(a): The optimum temperature of GAD activity;(b): temperature stability of GAD activity
(a): The optimum temperature of GAD activity;(b): temperature stability of GAD activity
Fig. 7. FT-IR spectrum of GAD1 and GAD2
Fig. 8. Raman spectrum of GAD1 and GAD2
Fig. 9. Fluorescence spectrum of GAD1 and GAD2
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Table 1. Effect of chemical reagents on GAD activity
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Table 2. Secondary structure of GAD1 and GAD2 determined by FT-IR
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Table 3. Secondary structure content of GAD1 and GAD2 determined by Raman
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Table 4. Side chain group band intensity of GAD1 and GAD2
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