We present a scheme for estimating the noise-equivalent temperature difference (NETD) of frequency upconversion detectors (UCDs) that detect mid-infrared (MIR) light. In particular, we investigate the frequency upconversion of a periodically poled crystal based on lithium niobate, where an MIR conversion bandwidth of 220 nm can be achieved in a single-poled period by a special design. Experimentally, for an MIR radiating target at a temperature of 95°C, the NETD of the device was estimated to be 56 mK with an exposure time of 1 s. Meanwhile, a direct measurement of the NETD was performed utilizing conventional methods, which resulted in 48 mK. We also compared the NETD of our UCD with commercially available direct MIR detectors. We show that the limiting factor for further NETD reduction of our device is not primarily from the upconversion process and camera noise but from the limitations of the heat source and laser performance. Our detectors have good temperature measurement performance and can be used for a variety of applications involving temperature object identification and material structure detection.