Compared with silica fibers, soft-glass fibers have advantages of high refractive index, high nonlinearity, and wide band transmission. Therefore, soft-glass fibers have a wide applications in fiber lasers, super continuous spectrum generation, and biosensors. In recent years, with the development of researches, soft-glass fibers exhibit excellent sensing performance in biosensing fields; specially, they exhibit high sensitivity and multipurpose sensing capability in near-infrared and mid-infrared bands. Therefore, they have various applications in the sensing field. In this study, basic characteristics, preparation methods, and applications in biosensing fields of soft-glass fibers are reviewed from the perspectives of temperature sensing, concentration sensing, gas sensing, and disease detection, and the future application prospect of soft-glass fibers is forecasted.