• Advanced Photonics
  • Vol. 3, Issue 2, 026004 (2021)
Herve Hugonnet1、2, Yeon Wook Kim3, Moosung Lee1、2, Seungwoo Shin1、2, Ralph H. Hruban4, Seung-Mo Hong4、5、*, and YongKeun Park1、2、6、*
Author Affiliations
  • 1Korea Advanced Institute of Science and Technology, Department of Physics, Daejeon, Republic of Korea
  • 2KAIST Institute for Health Science and Technology, KAIST, Daejeon, Republic of Korea
  • 3Asan Institute for Life Science, Asan Medical Center, Seoul, Republic of Korea
  • 4Johns Hopkins Medical Institutions, Sol Goldman Pancreatic Cancer Research Center, Department of Pathology, Baltimore, Maryland, United States
  • 5University of Ulsan College of Medicine, Asan Medical Center, Department of Pathology, Seoul, Republic of Korea
  • 6Tomocube Inc., Daejeon, Republic of Korea
  • show less

    Abstract

    Histopathology relies upon the staining and sectioning of biological tissues, which can be laborious and may cause artifacts and distort tissues. We develop label-free volumetric imaging of thick-tissue slides, exploiting refractive index distributions as intrinsic imaging contrast. The present method systematically exploits label-free quantitative phase imaging techniques, volumetric reconstruction of intrinsic refractive index distributions in tissues, and numerical algorithms for the seamless stitching of multiple three-dimensional tomograms and for reducing scattering-induced image distortion. We demonstrated label-free volumetric imaging of thick tissues with the field of view of 2 mm × 1.75 mm × 0.2 mm with a spatial resolution of 170 nm × 170 nm × 1400 nm. The number of optical modes, calculated as the reconstructed volume divided by the size of the point spread function, was ~20 giga voxels. We have also demonstrated that different tumor types and a variety of precursor lesions and pathologies can be visualized with the present method.
    Copy Citation Text
    Herve Hugonnet, Yeon Wook Kim, Moosung Lee, Seungwoo Shin, Ralph H. Hruban, Seung-Mo Hong, YongKeun Park. Multiscale label-free volumetric holographic histopathology of thick-tissue slides with subcellular resolution[J]. Advanced Photonics, 2021, 3(2): 026004
    Download Citation
    Category: Research Articles
    Received: Nov. 4, 2020
    Accepted: Mar. 23, 2021
    Posted: May. 6, 2021
    Published Online: May. 6, 2021
    The Author Email: Hugonnet Herve (grougrou@kaist.ac.kr), Kim Yeon Wook (dusnrl486@naver.com), Lee Moosung (lkaamo@kaist.ac.kr), Shin Seungwoo (fire6516@kaist.ac.kr), Hruban Ralph H. (rhruban@jhmi.edu), Hong Seung-Mo (smhong28@gmail.com), Park YongKeun (yk.park@kaist.ac.kr)