The types and contents of mineral elements in medicinal plants are closely associated with their growth environment and pertinent development process. Meanwhile, they are also closely related to the product of medicinal substances and their clinical effects. Therefore, they are important indicators for the quality evaluation of Chinese medicinal materials, and it is worth exploring on the relationship of the characteristics regarding elements with the different environmental factors, such as climate, soil, hydrology and so on. In this study, the concentrations of 35 elements in Ca, K, Mg, Na, Fe, Sn, Be, As, Al, V, Sc, Cr, Mn, Co, Bi, Ga, Ni, La, Mo, Ag, Hg, Cu, Nb, Zn, Ge, Se, Tl, Cd, Sb, Ba, Y, Ti, Pb, Zr and Sr were determined by inductively coupled plasma-mass spectrometry and inductively coupled plasma-atomic emission spectrometry techniques, and analyzed using multivariate statistical methods including variance analysis, principal component analysis and factor analysis. The results showed that the method had good selectivity, accuracy, within-day precision, recovery and linearity in their established ranges, respectively. There was a significant difference for 33 elements out of 35 in A. argyi from different locations (p＞0.05). According to the results of PCA, 7 principal components with a cumulative contribution rate of 82.75% were extracted from 35 elements for further analysis. Also, the distribution of A. argyi samples from different locations were relatively concentrated and was feasible for classification and evaluation. The comprehensive evaluation function for the 7 selected elements was F=0.449 1F1＋0.118F2＋0.097 2F3＋0.055 5F4＋0.042 5F5+0.034 5F6+0.030 7F7. By calculating the scores of comprehensive factors, the total values of Qi ai (Qichun, Hubei province) and Bei ai (Anguo, Hebei province) are on top 2. The study established an accurate and efficient analytical method and comprehensive evaluation system for the mineral elements in A. argyi from different ecological areas, provide a scientific basis for quality control of beneficial supplements in A. argyi, and may be valuable for the similar studies for other medicinal species.