• Laser & Optoelectronics Progress
  • Vol. 58, Issue 19, 1917001 (2021)
Liangchuang Su and Tao Chen*
Author Affiliations
  • Faculty of Materials and Manufacturing, Beijing University of Technology, Beijing 100124, China
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    DOI: 10.3788/LOP202158.1917001 Cite this Article Set citation alerts
    Liangchuang Su, Tao Chen. Wireless Stimulation System with Multiple Channels and Independent Regulation for Optogenetics in Vitro[J]. Laser & Optoelectronics Progress, 2021, 58(19): 1917001 Copy Citation Text show less

    Abstract

    To provide a larger area and larger regional selectivity of light stimulation in the application of optogenetics, a multi-channel optogenetic stimulation system with a simple structure, wireless control of stimulation parameters and independent control was developed by our research group. A μLED chip was used as the light source, and lithography and micro-welding technology were used to fabricate a blue light stimulator with an array light source. The wireless control function of the stimulator was realised using the wireless communication module. The size and weight of the photic stimulator were 46 mm × 30 mm and 7.134 g, respectively. The results showed that the maximum output optical power of the optrode's surface was 24.6 mW, which met the requirements of activating neurones by ChR2. The adjustable range of the output optical frequency was 1-500 Hz, while the maximum output error was less than 1%. The adjustable range of the duty cycle was 0-100%, while the maximum distance of the wireless communication was 50 m. This system can be used in the fields of optogenetic regulation, cell culture and fields that require a large area of light. Finally, it provides an effective tool for the regulation and research of neural circuits.
    Liangchuang Su, Tao Chen. Wireless Stimulation System with Multiple Channels and Independent Regulation for Optogenetics in Vitro[J]. Laser & Optoelectronics Progress, 2021, 58(19): 1917001
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