Sample-sample (s-s) and wavenumber-wavenumber (w-w) two-dimensional (2D) correlation analysis of Fourier-transform infrared (FT-IR) spectra were employed to investigate the thermal dynamics of bovine serum albumin (BSA) in the aqueous solution. S-s 2D correlation spectra show the temperature profile of BSA, which indicates that the drastic variations in BSA structure starts at 66 ℃. However, w-w 2D correlation spectra reveal the variations in the structure of BSA and their orders of variation in the process of temperature elevation. The study demonstrates the key roles of 2D correlation spectra in the analysis of thermal dynamics of protein.