Developing highly efficient and low-cost catalysts is crucial in the field of clean energy economy, in which ammonia borane (AB) has attracted great attention due to its high hydrogen production through the catalytic hydrolysis. In this work, BiVO₄ nanosheet was firstly synthesized by a facile reflux method. And then bimetallic RuFe@BiVO₄ catalysts with different molar ratios of Ru/Fe were prepared via in situ impregnation-reduction techniques and their catalytic activities were also tested in the hydrogen generation from aqueous solution of AB at room temperature. Compared with the releasing hydrogen rates of catalysts of BiVO₄, Ru@BiVO₄, Fe@BiVO₄, RuFe NPs and RuFe@BiVO₄, respectively, Ru₁Fe_0.1@BiVO₄ exhibits the highest catalytic activity for the dehydrogenation of AB among all catalysts, the activation energy (E_a) and turnover frequency (TOF) are 43.7 kJ·mol^-1 and 205.4 mol_H2·mol_Ru·min^-1, respectively. The addition of non-noble Fe can significantly enhance the catalytic activity of Ru counterparts, which is closely related to the strong electronic effect between Ru and Fe NPs, bi-functional effect generated between the RuFe NPs and the support BiVO₄.