• Spectroscopy and Spectral Analysis
  • Vol. 41, Issue 2, 419 (2021)
Yan-yan LIU1、1、*, Ning-ping TAO1、1, Xi-chang WANG1、1, Ying LU1、1, and Chang-hua XU1、1
Author Affiliations
  • 1[in Chinese]
  • 11. College of Food Sciences & Technology, Shanghai Ocean University, Shanghai 201306, China
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    DOI: 10.3964/j.issn.1000-0593(2021)02-0419-06 Cite this Article
    Yan-yan LIU, Ning-ping TAO, Xi-chang WANG, Ying LU, Chang-hua XU. Integrated Detection of Foodborne Pathogens by In-Situ Infrared Spectroscopy Based on ZnSe Film Transmission Method[J]. Spectroscopy and Spectral Analysis, 2021, 41(2): 419 Copy Citation Text show less
    Diagram (including sample pretreatment) of ZnSe film transmission and KBr pressed pellet method
    Fig. 1. Diagram (including sample pretreatment) of ZnSe film transmission and KBr pressed pellet method
    Integrated infrared macroscopic fingerprint of four foodborne pathogens based on ZnSe film transmission method
    Fig. 2. Integrated infrared macroscopic fingerprint of four foodborne pathogens based on ZnSe film transmission method
    Comparison of IR spectra in four foodborne pathogens based on ZnSe film transmission and KBr pressed pellet method(a): E. coli DH5α; (b): S. enteritidis CMCC 50041; (c): V. cholerae SH04; (d): S. aureus SH10
    Fig. 3. Comparison of IR spectra in four foodborne pathogens based on ZnSe film transmission and KBr pressed pellet method
    (a): E. coli DH5α; (b): S. enteritidis CMCC 50041; (c): V. cholerae SH04; (d): S. aureus SH10
    SD-IR spectra of E. coli DH5α in the region of 4 000~600 cm-1
    Fig. 4. SD-IR spectra of E. coli DH5α in the region of 4 000~600 cm-1
    Comparison of SD-IR comparison spectra in four foodborne pathogens based on ZnSe film transmission and KBr pressed pellet method (1 500~900 cm-1) (a): E. coli DH5α; (b): S. enteritidis CMCC 50041; (c): V. cholerae SH04; (d): S. aureus SH10
    Fig. 5. Comparison of SD-IR comparison spectra in four foodborne pathogens based on ZnSe film transmission and KBr pressed pellet method (1 500~900 cm-1)
    (a): E. coli DH5α; (b): S. enteritidis CMCC 50041; (c): V. cholerae SH04; (d): S. aureus SH10
    Wavenumber/cm-1Spectral regionBand assignment
    3 000~2 800Lipidsνas/s(CH3), νas/s(CH2)
    1 800~1 500Proteins and peptidesAmide Ⅰ, Amide Ⅱ
    1 500~1 200A mixed regionδs(CH2), νs(COO-), Amide Ⅲ, v85(P=O)
    1 200~900PolysaccharidesC—O—C, C—O ring vibrations
    900~700A fingerprint region-
    Table 1. Major spectral band assignments for Fourier transform infrared spectroscopy (FT-IR) of four foodborne pathogens
    Bacterial strainsZnSe film transmission methodKBr pressed pellet method
    Number of
    labeled peaks
    Number of
    strong peaks
    Number of
    twin peaks
    Number of
    labeled peaks
    Number of
    strong peaks
    Number of
    twin peaks
    E. coli DH5α1912412--
    S. enteritidis CMCC 50041208314--
    V. cholerae SH0422721731
    S. aureus SH1020113153-
    Table 2. Comparison of characteristic peak in SD-IR spectra (1 500~900 cm-1) based on ZnSe film transmission and KBr pressed pellet method
    Yan-yan LIU, Ning-ping TAO, Xi-chang WANG, Ying LU, Chang-hua XU. Integrated Detection of Foodborne Pathogens by In-Situ Infrared Spectroscopy Based on ZnSe Film Transmission Method[J]. Spectroscopy and Spectral Analysis, 2021, 41(2): 419
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