A cavity-stabilized 578 nm laser is used to probe the clock transition of ytterbium atoms trapped in optical lattice sites. We obtain a Fourier-limited 4.2-Hz-linewidth Rabi spectrum and a Ramsey spectrum with fringe linewidth of 3.3 Hz. Based on one of the spectra, the 578 nm laser light is frequency-stabilized to the center of the transition to achieve a closed-loop operation of an optical clock. Based on interleaved measurement, the frequency instability of a single optical clock is demonstrated to be 5.4 × 10^-16/√τ.